Glyphosate, the most used herbicide in the World, has been found in the urine of 93% of the American public during a unique testing project that started in 2015.

Glyphosate, labeled a ‘probable human carcinogen’ by the World Health Organization’s cancer agency IARC in 2015, has now been revealed to be ubiquitous in the first ever comprehensive and validated LC/MS/MS testing project to be carried out across America.

The European Union is currently in the process of putting restrictions on the use of glyphosate due to health concerns, with Member States so far unable to agree on the re-approval of the chemical beyond June 2016.

Glyphosate-containing herbicides are sold under trademarks such as Monsanto’s ‘Roundup’.

Summary of Testing

• Urine and Water Testing Results

93% of the urine tested by the University of California San Francisco lab tested positive for glyphosate residues. No glyphosate was found in the tap water samples. These results are only from a small percentage of the total samples collected – more data will be released later in 2016.

The results of this bio-survey come from the first in-lab validated LC/MS/MS testing method used for glyphosate testing of the general public in America.

• Is Glyphosate Safe at Real-Life Exposure Levels?:

Glyphosate has never been studied by regulators or the chemical industry at levels that the human population in the U.S. is being exposed to (under 3 mg/kg body weight/day). This is a huge hole in the global risk assessment of glyphosate, as there is evidence suggesting that low levels of the chemical may hack hormones even more than at mid and high levels, according to independent science – a higher dose does not necessarily make a more toxic, hormone disruptive effect.

• Unique Testing Project

The Detox Project is a unique platform for the testing of toxic chemicals in our bodies and in our food. The aim of the project is to cut out all conflicts of interest in chemical bio-surveys by allowing the public to pay directly for chemical testing that they are interested in.

Urine and Water Testing Results

In a unique public testing project carried out by a laboratory at the University of California San Francisco (UCSF), glyphosate was discovered in 93% of urine samples during the early phase of the testing in 2015.

The urine and water testing was organized by The Detox Project and commissioned by the Organic Consumers Association.

The unique project, which has already provided more urine samples for testing than any other glyphosate bio-monitoring urine study ever in America, was supported by members of the public, who themselves paid for their urine and water samples to be analyzed for glyphosate residues by the UCSF lab.

The data released in a presentation by the UCSF lab only covers the first 131 people tested. Further data from this public bio-monitoring study, which is now completed, will be released later in 2016.

The Detox Project will be working alongside a new larger lab later this year to enable the public to once again test their urine for glyphosate residues.

The UCSF full presentation of these initial results can be found here.

The Results

Glyphosate was found in 93% of the 131 urine samples tested at an average level of 3.096 parts per billion (PPB). Children had the highest levels with an average of 3.586 PPB.

The regions with the highest levels were the West and the Midwest with an average of 3.053 PPB and 3.050 PPB respectively.

Glyphosate residues were not observed in any tap water samples during the early phase of the project, most likely due to phosphorus removal during water treatment.

The results from the UCSF urine testing in America showed a much higher frequency and average glyphosate level than those observed in urine samples in the European Union in 2013. The average level in Europe was around 1 PPB with a frequency of detection of 43.9%.

The Method

Glyphosate (N-(phosphonomethyl)glycine ) is directly analyzed using liquid chromatography- tandem mass spectrometry (LC-MS/MS). Water and urine samples are prepared for analysis by solid phase extraction using an ion exchange column. Extracted samples are injected to the LC-MS/MS and the analyte is separated using an Obelisc N column (SIELC Technologies, Prospect Heights, IL) through isocratic elution. Ionization of glyphosate is achieved using an electrospray ionization source operated in negative polarity. The analyte is detected by multiple reaction monitoring using a 13C-labelled glyphosate as internal standard. Quantification of the analyte is done by isotope dilution method using an eight-point calibration curve.

The assay has a limit of quantification of 0.5 ng/mL. The intra- and inter-day precision observed are 6-15% in concentrations that range 0.5-80 ng/mL. Recoveries for glyphosate range 70-80% at concentrations within the assay’s linear dynamic range.

Contact at UCSF lab:  Dr. Roy Gerona – Roy.Gerona@ucsf.edu

Is Glyphosate Safe at Real-Life Exposure Levels?

Glyphosate has never been studied by regulators or the chemical industry at levels that the human population in the U.S. is being exposed to (under 3 mg/kg body weight/day). This is a huge hole in the risk assessment process for glyphosate, as evidence suggests that low levels of the chemical may hack hormones even more than high levels – a higher dose does not necessarily mean a more toxic, hormone disruptive effect.

Industry funded science from the dark ages suggested that the higher the dose of a chemical the more dangerous it was. However, modern independent science has discovered that many toxic chemicals have as much or even more of an influence on our health at low doses– these chemicals are known as hormone hackers (endocrine disruptors).

A study from March 2015 stated that the health costs to the European Union of just some hormone hacking chemicals, in connection with a subset of illnesses known to be linked to hormone interference, is over EUR 150 Billion per year! The study stated that lower IQ, adult obesity and 5% or more of autism cases are all linked to exposure to endocrine disruptors.

Glyphosate is likely to be one of these hormone hacking chemicals at real-life exposure levels down to 0.1 ppb or below, according to independent science. Regulators and the chemical industry have simply not studied the toxic effects of glyphosate at real-life exposure levels. Find more information on this here.

More information: How safe are “safe” levels of glyphosate?

Scientist Contacts:

Regarding Real-Life Exposure Levels of Glyphosate:

Dr. Pete Myers, Founder, CEO and Chief Scientist of Environmental Health Sciences – JPMyers@ehsic.org

Dr. Michael Antoniou, molecular geneticist, London, UK – michael.antoniou@kcl.ac.uk

“With increasing evidence from laboratory studies showing that glyphosate based herbicides can result in a wide range of chronic illnesses through multiple mechanisms, it has become imperative to ascertain the levels of glyphosate in food and in as large a section of the human population as possible. Thus the information gathered by the glyphosate public testing service being offered by the Detox Project is most timely and will provide invaluable information for the consumer and scientists like myself evaluating the toxicity of real world levels of exposure to this most widely used pesticide.”

For more comments on the Testing Results contact:

Henry Rowlands, Director, The Detox Project – henry@detoxproject.org   

 “These results show that both the U.S. regulators have let down consumers in America. Independent science shows that glyphosate may be a hormone hacker at these real-life exposure levels found in the food products. The safe level of glyphosate ingestion is simply unknown despite what the EPA and Monsanto would have everyone believe.”

“The Detox Project which organized this testing is a research platform that brings awareness to the public by testing for toxic chemicals in our bodies and in our food at a very personal level. We believe everyone has the right to know what man-made toxic chemicals are in our bodies and in our food.”

Ronnie Cummins, International Director, Organic Consumers Association – ronnie@organicconsumers.org

“If consumers had any doubt about the extent to which they are being poisoned by Monsanto’s Roundup, these tests results should put those doubts to rest.”

“These test results highlight the massive failure of U.S. regulatory agencies, including the EPA, USDA and FDA, to protect us even as they continue to perpetuate the myth that low-level exposure to glyphosate is harmless. We must bring down this poison-for-profit model and build in its place a food and farming system that regenerates and heals, before it’s too late.”